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Protocols

The first early blight subgroup meeting brought together 19 Euroblight members on 19-20 march at Freising, Germany. Over the two days EB field experiments and fungicide ratings were discussed along with increasing problems associated with losses in fungicide sensitivity. In order to improve and standardize the monitoring and diagnostics of EB relevant species lab protocols for testing and comparison are now available on the EuroBlight website. Protocols collated by the Alternaria sub-group is organized according to different topics and they will be further elaborated and new ones will appear whenever appropriate.  

Protocol title

Contact person

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qPCR

Qualitative PCR diagnostics of A. solani and A. alternata

Andrea Volz, a.backhaus@wzw.tum.de;
Jürgen Leiminger, juergen.leiminger@lfl.bayern.de
Lehrstuhl für Phytopathologie,
Wissenschaftszentrum Weihenstephan
Emil-Ramann-Str. 2
85354-Freising

June 2014

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Artificial inoculation

Artificial inoculation with Alternaria solani & Alternaria alternata (detached leaf, greenhouse and field)

Jan Spoelder, Spoelder@hlbbv.nl
HLB
Diagnostics Dept.
Kampsweg 27
9418 PD Wijster
The Netherlands

June 2014

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Artificial inoculation - Greenhouse studies

Dr. Birgit Adolf, adolf@wzw.tum.de
Lehrstuhl für Phytopathologie, Wissenschaftszentrum Weihenstephan, Emil-Ramann-Str. 2. 85354 Freising

June 2014

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Methods for inoculation of tomatoes and potatoes with Alternaria solani in the greenhouse and field

Gerd Stammler, Simone Miessner, Franziska Böhme, gerd.stammler@basf.com.

BASF SE, Agricultural Center, Speyerer Strasse 2, 67117 Limburgerhof, Germany

June 2014

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Protocol for the artificial inoculation with A. solani in field trials (with infected kernels)

Birgit Adolf, Hans Hausladen

Lehrstuhl für Phytopathologie, Wissenschaftszentrum Weihenstephan, Emil-Ramann-Str. 2. 85354 Freising, Germany. adolf@wzw.tum.de

July 2015

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Long-Term Storage

Long-term storage of Alternaria isolates

Andrea Volz, a.backhaus@wzw.tum.de;
Jürgen Leiminger, juergen.leiminger@lfl.bayern.de
Lehrstuhl für Phytopathologie,
Wissenschaftszentrum Weihenstephan
Emil-Ramann-Str. 2
85354-Freising

June 2014

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Long term storage of Alternaria isolates

Bert Evenhuis
bert.evenhuis@wur.nl
Wageningen UR PPO-AGV-Plant Research International
PO BOX 16
6700 AA Wageningen

June 2014

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Long term storage of Alternaria isolates

Jan Spoelder,
Spoelder@hlbbv.nl
HLB
Diagnostics Dept.
Kampsweg 27
9418 PD Wijster
The Netherlands

June 2014

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Growth and conidia production

Growth and conidia production of Alternaria solani

Jan Spoelder,
Spoelder@hlbbv.nl
HLB
Diagnostics Dept.
Kampsweg 27
9418 PD Wijster
The Netherlands

June 2014

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Alternaria solani growth and spore production protocol

Jürgen Leiminger, juergen.leiminger@lfl.bayern.de
Lehrstuhl für Phytopathologie,
Wissenschaftszentrum Weihenstephan
Emil-Ramann-Str. 2
85354-Freising

June2014

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Protocol for conidia production of Alternaria solani

Bert Evenhuis
bert.evenhuis@wur.nl
Wageningen UR PPO-AGV-Plant Research International
PO BOX 16
6700 AA Wageningen

June 2014

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Protocol for growth and conidia production of Alternaria solani

Firuz Odilbekov and Erland Liljeroth, Erland.Liljeroth@slu.se
Swedish University of Agricultural Sciences

June2014

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Isolation

Isolation of A. solani

Jürgen Leiminger, juergen.leiminger@lfl.bayern.de
Lehrstuhl für Phytopathologie,
Wissenschaftszentrum Weihenstephan
Emil-Ramann-Str. 2
85354-Freising

June 2014

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Screening for Alternaria solani or alternata

Bert Evenhuis
bert.evenhuis@wur.nl
Wageningen UR PPO-AGV-Plant Research International
PO BOX 16
6700 AA Wageningen

June 2014

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Characterization of Cytb mutations

Identification of Alternaria solani GI in combination with analysis of the F129L substitution

Eva Edin, eva.edin@slu.se,
Dept. Forest Mycology & Plant Pathology, Swedish University of Agricultural Sciences, P.O. Box 7026, SE 75007 Uppsala Sweden

June 2014

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Protocol for the detection of a G143A mutation in Alternaria alternata-isolates

Jan Nechwatal,
jan.nechwatal@lfl.bayern.de
Bayerische Landesanstalt für Landwirtschaft
Institut für Pflanzenschutz
Lange Point 10
85354 Freising

June 2014

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Identification of Alternaria alternata in combination with analysis of the G143A substitution

Eva Edin, eva.edin@slu.se,
Dept. Forest Mycology & Plant Pathology, Swedish University of Agricultural Sciences, P.O. Box 7026, SE 75007 Uppsala Sweden

June 2014

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Prevalence of the F129L mutation in Alternaria solani from the United States

Neil Gudmestad, Neil.Gudmestad@ndsu.edu,
Department of Plant Pathology
Dept 7660, PO Box 6050  /  Fargo, ND  58108-6050

June 2014

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Confirmation of resistant phenotypes by the proof of cytb mutations (F129L, G143A) in Alternaria isolates

 

Dr. Birgit Adolf, adolf@wzw.tum.de
Lehrstuhl für Phytopathologie, Wissenschaftszentrum Weihenstephan, Emil-Ramann-Str. 2. 85354 Freising

June 2014

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Revised 01.07.2015